谢剑平院士：碳纳米颗粒促烟草钾吸收机制 | NMT烟草品质创新科研平台

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研究使用平台：NMT烟草品质创新科研平台

期刊：Journal of Nanobiotechnology
       主题：碳纳米颗粒促烟草钾吸收机制
       标题：Carbon nanoparticles enhance potassium uptake via upregulating potassium channel expression and imitating biological ion channels in BY‑2 cells
       影响因子：5.345
       检测指标：K+流速
       检测样品：烟草

NO3-流实验处理方法：
烟草BY-2悬浮细胞，分别在62.50μg/mL CNP，125.00μg/mL CNP和125.00μg/mL CNP+10mM TEA环境下处理12小时
       NO3-流实验测试液成份：
46.88 mM KCl, 0.1 mM CaCl2, 0.3 mM MES, pH 5.9
       作者：中国烟草总公司郑州烟草研究院谢剑平、陈丽娟

中文摘要（谷歌机翻）

碳纳米颗粒（CNPs）可以促进植物生长，而到目前为止，尚未报道CNPs促进植物生长吸收钾的机制。

在这项研究中，建立了CNPs促进BY-2细胞吸收钾的功能，并且细胞中积累的钾与细胞的新鲜生物量具有显着相关性。细胞中的K+随着CNP浓度的增加而增加。用CNP处理后，K+流入量达到高水平，并且显着高于用K+阻断剂氯化四乙铵（TEA+）处理的对照组和阴性组。

在CNPs抑制剂存在下，K+积累没有减少。与对照组相比，在钾通道阻滞剂TEA+或CNPs抑制剂的存在下，NKT1基因表达发生了变化。发现CNP比圆锥形纳米毛细管中通过离子电流测定法（RIC）整流确定的其他阳离子优先运输K+。

这些结果表明CNPs上调钾基因表达以增强BY-2细胞中的K+。此外，据推测，CNP通过大量的羧基模拟了离子通道的蛋白质，以渗透K+。这些发现将为通过碳纳米颗粒改善植物生长提供支持。

Effects of CNPs and inhibitor treatments on net K+ fluxes in BY-2 cells. a K fluxes were determined with after different CNPs treatments lasting for 900 s measurements. b Schematic diagram of test sample. c Mean value of K+ fluxes at different concentration of CNPs in cells. Group 1: 62.50 μg/mL CNPs, Group 2: 125.00 μg/mL CNPs, Group 3: 125.00 μg/mL CNPs + 10 mM TEA+. The mean value of each group contains six individual cell, and error bars represent SE, n = 6. Asterisk (*) indicates significant difference compared with control group (p < 0.05)

英文摘要

Carbon nanoparticles (CNPs) have been reported to boost plant growth, while the mechanism that CNPs enhanced potassium uptake for plant growth has not been reported so far.

In this study, the function that CNPs promoted potassium uptake in BY-2 cells was established and the potassium accumulated in cells had a significant correlation with the fresh biomass of  cells. The K+ in cells increased with the increasing concentration of CNPs. The K+ influx reached high level after treatment with CNPs and was significantly higher than that of the control group and the negative group treated with K+ blocker, tetraethylammonium chloride ( TEA+).

The K+ accumulation was not reduced in the presence of CNPs inhibitors. In the presence of potassium channel blocker TEA+ or CNPs inhibitors, the NKT1 gene expression was changed compared with the control group. The CNPs were found to preferentially transport K+ than other cations determined by rectification of ion current assay (RIC) in a conical nanocapillary.

These results indicated that CNPs upregulated potassium gene expression to enhance K+ in BY-2 cells. Moreover, it was speculated that the CNPs simulated protein of ion channels via bulk of carboxyl for K+ permeating. These findings will provide support for improving plant growth by carbon nanoparticles.