期刊：JOURNAL OF AGRICULTURALAND FOOD CHEMISTRY
标题：Low Nitrogen Enhances Nitrogen Use Efficiency by Triggering NO3– Uptake and Its Long-Distance Translocation
Nitrogen is essential for plant growth andcrop productivity; however, nitrogen use eﬃciency (NUE) decreases withincreasing N supply, resulting in a waste of resources. Molecular mechanismsunderlying low-nitrogen (LN)-mediated enhancement of NUE are not clear.
We used high-NUE Brassica napus genotype H(Xiangyou 15), low-NUE B. napus genotype L (814), and Arabidopsis mutant aux1to elucidate the mechanism underlying the changes in NUE under diﬀerent ratesof N fertilizer application. NUE of B. napus increased under LN, which enhancedN uptake ability by regulating root system architecture and plasma membraneH+-ATPase activity; AUX1 was involved in this process.
Additionally, BnNRT1.5 was upregulated andBnNRT1.8 was downregulated under LN, whereby more N was transferred to theshoot through enhanced N transport. Observed changes in photosynthesis under LNwere associated with N assimilation eﬃciency. Our study provides new insightsinto the mechanisms of plant adaptation to the environment.
我们使用高NUE甘蓝型油菜基因型H（香优15），低NUE B.napus基因型L（814）和拟南芥突变体aux1来阐明在不同氮肥施用量下NUE变化的机制。甘蓝型油菜NUE在LN下增加，通过调节根系结构和质膜H + -ATPase活性提高N吸收能力; AUX1参与了这个过程。
Gas exchange, chlorophyll content, and photosynthetic N use in two contrasting B. napus genotypes for N use eﬃciency under LN (1 mM NO3− N, black bars) or CK (15 mM NO3− N, white bars) conditions: (a) photosynthetic rate, (b) SPAD, (c) transpiration rate, and (d) photosynthetic N use eﬃciency (PNUE). The bar height represents the mean, and error bars indicate standard deviation (n = 6). Diﬀerent letters on bars indicate signiﬁcant diﬀerences based on Tukey’s HSD (p < 0.05). The statistical analysis was performed on diﬀerent treatments within genotypes.
· V-ATPase and V-PPase at the Tonoplast Affect NO3- Content in Brassica napus by Controlling Distribution of NO3- Between the Cytoplasm and Vacuole
· Nitrogen Use Efficiency Is Mediated by Vacuolar Nitrate Sequestration Capacity in Roots of Brassica napus
· NRT1.1-related NH4+ toxicity is associated with balance between NH4+ uptake and assimilation
（一作视频讲解）J Agr Food Chem：湖南农大 | 低氮提升油菜氮利用率